c1 preloading Search Results


piercetm high capacity neutravidintm agarose  (Thermo Fisher)
99
Thermo Fisher piercetm high capacity neutravidintm agarose
Piercetm High Capacity Neutravidintm Agarose, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c1+preloading/10__1074_slash_jbc__ra119__008762-332-40-47?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
piercetm high capacity neutravidintm agarose - by Bioz Stars, 2026-07
99/100 stars
  Buy from Supplier
glutathione sepharose 4b  (Cytiva Europe)
99
Cytiva Europe glutathione sepharose 4b
Glutathione Sepharose 4b, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c1+preloading/bio_rxiv__2021__10__21__465321-237-11-14?v=Cytiva+Europe
Average 99 stars, based on 1 article reviews
glutathione sepharose 4b - by Bioz Stars, 2026-07
99/100 stars
  Buy from Supplier
preload affi gel agarose beads  (Bio-Rad)
94
Bio-Rad preload affi gel agarose beads
Preload Affi Gel Agarose Beads, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c1+preloading/pm18572158-38-23-30?v=Bio-Rad
Average 94 stars, based on 1 article reviews
preload affi gel agarose beads - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier
glutathione sepharose 4b beads  (GE Healthcare)
99
GE Healthcare glutathione sepharose 4b beads
Confirmation of protein-protein interactions identified by the screens. A, Fluorescent microscopy images of bait-prey protein pairs tagged with YFP fragments. HTC75 cells stably coexpressing YFPn-tagged TRF1 and YFPc alone (negative control), YFPc-tagged TIN2 (positive control), YFPc-MDH1, or YFPc-SET were visualized live under a fluorescence microscope. Hoechst33342 was added to visualize the nuclei. B, A flow chart of secondary coprecipitation screens that were used to confirm the identified protein-protein interactions. C, Examples of coprecipitation screens. Cell extracts from 293T cells coexpressing FLAG-tagged candidate proteins with GST alone or GST-tagged telomeric bait proteins were incubated with <t>GSH-agarose</t> beads. The proteins bound to <t>GSH</t> <t>beads</t> were then dot-blotted and probed with anti-FLAG antibodies.
Glutathione Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c1+preloading/pmc03033672-214-20-26?v=GE+Healthcare
Average 99 stars, based on 1 article reviews
glutathione sepharose 4b beads - by Bioz Stars, 2026-07
99/100 stars
  Buy from Supplier
preload affi gel blue agarose beads  (Bio-Rad)
96
Bio-Rad preload affi gel blue agarose beads
Confirmation of protein-protein interactions identified by the screens. A, Fluorescent microscopy images of bait-prey protein pairs tagged with YFP fragments. HTC75 cells stably coexpressing YFPn-tagged TRF1 and YFPc alone (negative control), YFPc-tagged TIN2 (positive control), YFPc-MDH1, or YFPc-SET were visualized live under a fluorescence microscope. Hoechst33342 was added to visualize the nuclei. B, A flow chart of secondary coprecipitation screens that were used to confirm the identified protein-protein interactions. C, Examples of coprecipitation screens. Cell extracts from 293T cells coexpressing FLAG-tagged candidate proteins with GST alone or GST-tagged telomeric bait proteins were incubated with <t>GSH-agarose</t> beads. The proteins bound to <t>GSH</t> <t>beads</t> were then dot-blotted and probed with anti-FLAG antibodies.
Preload Affi Gel Blue Agarose Beads, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c1+preloading/pmc01850842-51-54-67?v=Bio-Rad
Average 96 stars, based on 1 article reviews
preload affi gel blue agarose beads - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
ni sepharose-preloaded column  (Promega)
90
Promega ni sepharose-preloaded column
Confirmation of protein-protein interactions identified by the screens. A, Fluorescent microscopy images of bait-prey protein pairs tagged with YFP fragments. HTC75 cells stably coexpressing YFPn-tagged TRF1 and YFPc alone (negative control), YFPc-tagged TIN2 (positive control), YFPc-MDH1, or YFPc-SET were visualized live under a fluorescence microscope. Hoechst33342 was added to visualize the nuclei. B, A flow chart of secondary coprecipitation screens that were used to confirm the identified protein-protein interactions. C, Examples of coprecipitation screens. Cell extracts from 293T cells coexpressing FLAG-tagged candidate proteins with GST alone or GST-tagged telomeric bait proteins were incubated with <t>GSH-agarose</t> beads. The proteins bound to <t>GSH</t> <t>beads</t> were then dot-blotted and probed with anti-FLAG antibodies.
Ni Sepharose Preloaded Column, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c1+preloading/pmc05431462-251-68-71?v=Promega
Average 90 stars, based on 1 article reviews
ni sepharose-preloaded column - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
c1 preloading  (fluidigm)
96
fluidigm c1 preloading
Confirmation of protein-protein interactions identified by the screens. A, Fluorescent microscopy images of bait-prey protein pairs tagged with YFP fragments. HTC75 cells stably coexpressing YFPn-tagged TRF1 and YFPc alone (negative control), YFPc-tagged TIN2 (positive control), YFPc-MDH1, or YFPc-SET were visualized live under a fluorescence microscope. Hoechst33342 was added to visualize the nuclei. B, A flow chart of secondary coprecipitation screens that were used to confirm the identified protein-protein interactions. C, Examples of coprecipitation screens. Cell extracts from 293T cells coexpressing FLAG-tagged candidate proteins with GST alone or GST-tagged telomeric bait proteins were incubated with <t>GSH-agarose</t> beads. The proteins bound to <t>GSH</t> <t>beads</t> were then dot-blotted and probed with anti-FLAG antibodies.
C1 Preloading, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c1+preloading/bio_rxiv__2025__05__30__657016-113-25-32?v=fluidigm
Average 96 stars, based on 1 article reviews
c1 preloading - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
wang polystyrene resin  (Chem Impex International)
95
Chem Impex International wang polystyrene resin
Confirmation of protein-protein interactions identified by the screens. A, Fluorescent microscopy images of bait-prey protein pairs tagged with YFP fragments. HTC75 cells stably coexpressing YFPn-tagged TRF1 and YFPc alone (negative control), YFPc-tagged TIN2 (positive control), YFPc-MDH1, or YFPc-SET were visualized live under a fluorescence microscope. Hoechst33342 was added to visualize the nuclei. B, A flow chart of secondary coprecipitation screens that were used to confirm the identified protein-protein interactions. C, Examples of coprecipitation screens. Cell extracts from 293T cells coexpressing FLAG-tagged candidate proteins with GST alone or GST-tagged telomeric bait proteins were incubated with <t>GSH-agarose</t> beads. The proteins bound to <t>GSH</t> <t>beads</t> were then dot-blotted and probed with anti-FLAG antibodies.
Wang Polystyrene Resin, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c1+preloading/pmc09827630-250-30-32?v=Chem+Impex+International
Average 95 stars, based on 1 article reviews
wang polystyrene resin - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier
protein g sepharose  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology protein g sepharose
Confirmation of protein-protein interactions identified by the screens. A, Fluorescent microscopy images of bait-prey protein pairs tagged with YFP fragments. HTC75 cells stably coexpressing YFPn-tagged TRF1 and YFPc alone (negative control), YFPc-tagged TIN2 (positive control), YFPc-MDH1, or YFPc-SET were visualized live under a fluorescence microscope. Hoechst33342 was added to visualize the nuclei. B, A flow chart of secondary coprecipitation screens that were used to confirm the identified protein-protein interactions. C, Examples of coprecipitation screens. Cell extracts from 293T cells coexpressing FLAG-tagged candidate proteins with GST alone or GST-tagged telomeric bait proteins were incubated with <t>GSH-agarose</t> beads. The proteins bound to <t>GSH</t> <t>beads</t> were then dot-blotted and probed with anti-FLAG antibodies.
Protein G Sepharose, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c1+preloading/pm22308310-88-22-15?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
protein g sepharose - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
gammabind-plus-sepharose beads  (Pharmacia LKB Biotechnology Inc)
90
Pharmacia LKB Biotechnology Inc gammabind-plus-sepharose beads
Confirmation of protein-protein interactions identified by the screens. A, Fluorescent microscopy images of bait-prey protein pairs tagged with YFP fragments. HTC75 cells stably coexpressing YFPn-tagged TRF1 and YFPc alone (negative control), YFPc-tagged TIN2 (positive control), YFPc-MDH1, or YFPc-SET were visualized live under a fluorescence microscope. Hoechst33342 was added to visualize the nuclei. B, A flow chart of secondary coprecipitation screens that were used to confirm the identified protein-protein interactions. C, Examples of coprecipitation screens. Cell extracts from 293T cells coexpressing FLAG-tagged candidate proteins with GST alone or GST-tagged telomeric bait proteins were incubated with <t>GSH-agarose</t> beads. The proteins bound to <t>GSH</t> <t>beads</t> were then dot-blotted and probed with anti-FLAG antibodies.
Gammabind Plus Sepharose Beads, supplied by Pharmacia LKB Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c1+preloading/pm09622505-119-38-26?v=Pharmacia+LKB+Biotechnology+Inc
Average 90 stars, based on 1 article reviews
gammabind-plus-sepharose beads - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


Confirmation of protein-protein interactions identified by the screens. A, Fluorescent microscopy images of bait-prey protein pairs tagged with YFP fragments. HTC75 cells stably coexpressing YFPn-tagged TRF1 and YFPc alone (negative control), YFPc-tagged TIN2 (positive control), YFPc-MDH1, or YFPc-SET were visualized live under a fluorescence microscope. Hoechst33342 was added to visualize the nuclei. B, A flow chart of secondary coprecipitation screens that were used to confirm the identified protein-protein interactions. C, Examples of coprecipitation screens. Cell extracts from 293T cells coexpressing FLAG-tagged candidate proteins with GST alone or GST-tagged telomeric bait proteins were incubated with GSH-agarose beads. The proteins bound to GSH beads were then dot-blotted and probed with anti-FLAG antibodies.

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Genome-wide YFP Fluorescence Complementation Screen Identifies New Regulators for Telomere Signaling in Human Cells *

doi: 10.1074/mcp.M110.001628

Figure Lengend Snippet: Confirmation of protein-protein interactions identified by the screens. A, Fluorescent microscopy images of bait-prey protein pairs tagged with YFP fragments. HTC75 cells stably coexpressing YFPn-tagged TRF1 and YFPc alone (negative control), YFPc-tagged TIN2 (positive control), YFPc-MDH1, or YFPc-SET were visualized live under a fluorescence microscope. Hoechst33342 was added to visualize the nuclei. B, A flow chart of secondary coprecipitation screens that were used to confirm the identified protein-protein interactions. C, Examples of coprecipitation screens. Cell extracts from 293T cells coexpressing FLAG-tagged candidate proteins with GST alone or GST-tagged telomeric bait proteins were incubated with GSH-agarose beads. The proteins bound to GSH beads were then dot-blotted and probed with anti-FLAG antibodies.

Article Snippet: The cleared lysate was then transferred into a 96-well binding plate (Purelink Clarificaiton plate, Invitrogen) preloaded with 100 μl/well of glutathione Sepharose 4B beads (10% slurry) (GE Healthcare Bio-Sciences AB), and incubated for 2 h at 4 °C with gentle agitation.

Techniques: Microscopy, Stable Transfection, Negative Control, Positive Control, Fluorescence, Incubation